Medical and Veterinary Entomology (2014) 28, 40-50

H. Xiong, D. Campelo, R.J. Pollack, D. Raoult, R. Shao, M. Alem, J. Ali, K. Bilcha and S.C. Barker (2014)
Second-generation sequencing of entire mitochondrial coding-regions (~15.4 kb) holds promise for study of the phylogeny and taxonomy of human body lice and head lice
Medical and Veterinary Entomology 28 (S1), 40-50
Abstract: The Illumina Hiseq platform was used to sequence the entire mitochondrial coding-regions of 20 body lice, Pediculus humanus Linnaeus, and head lice, P. capitis De Geer (Phthiraptera: Pediculidae), from eight towns and cities in five countries: Ethiopia, France, China, Australia and the U.S.A. These data (~310 kb) were used to see how much more informative entire mitochondrial coding-region sequences were than partial mitochondrial coding-region sequences, and thus to guide the design of future studies of the phylogeny, origin, evolution and taxonomy of body lice and head lice. Phylogenies were compared from entire coding-region sequences (~15.4 kb), entire cox1 (~1.5 kb), partial cox1 (~700 bp) and partial cytb (~600 bp) sequences. On the one hand, phylogenies from entire mitochondrial coding-region sequences (~15.4 kb) were much more informative than phylogenies from entire cox1 sequences (~1.5 kb) and partial gene sequences (~600 to ~700 bp). For example, 19 branches had > 95% bootstrap support in our maximum likelihood tree from the entire mitochondrial coding-regions (~15.4 kb) whereas the tree from 700 bp cox1 had only two branches with bootstrap support > 95%. Yet, by contrast, partial cytb (~600 bp) and partial cox1 (~486 bp) sequences were sufficient to genotype lice to Clade A, B or C. The sequences of the mitochondrial genomes of the P. humanus, P. capitis and P. schaeffi Fahrenholz studied are in NCBI GenBank under the accession numbers KC660761-800, KC685631-6330, KC241882-97, EU219988-95, HM241895-8 and JX080388-407.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Stephen C. Barker

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pediculus humanus		France
	Pediculus humanus		Ethiopia
	Pediculus humanus		China (NW)
	Pediculus humanus		Australia (NT+QLD)